Journal article
Deregulation of MYCN, LIN28B and LET7 in a molecular subtype of aggressive high-grade serous ovarian cancers
A Helland, MS Anglesio, J George, PA Cowin, CN Johnstone, CM House, KE Sheppard, D Etemadmoghadam, N Melnyk, AK Rustgi, WA Phillips, H Johnsen, R Holm, GB Kristensen, MJ Birrer, RB Pearson, AL Børresen-Dale, DG Huntsman, A deFazio, CJ Creighton Show all
Plos One | Published : 2011
Abstract
Molecular subtypes of serous ovarian cancer have been recently described. Using data from independent datasets including over 900 primary tumour samples, we show that deregulation of the Let-7 pathway is specifically associated with the C5 molecular subtype of serous ovarian cancer. DNA copy number and gene expression of HMGA2, alleles of Let-7, LIN28, LIN28B, MYC, MYCN, DICER1, and RNASEN were measured using microarray and quantitative reverse transcriptase PCR. Immunohistochemistry was performed on 127 samples using tissue microarrays and anti-HMGA2 antibodies. Fluorescence in situ hybridisation of bacterial artificial chromosomes hybridized to 239 ovarian tumours was used to measure trans..
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Awarded by United States Army Medical Research and Materiel Command
Funding Acknowledgements
AOCS was supported by the United States Army Medical Research and Materiel Command under DAMD17-01-1-0729, The Cancer Council Tasmania, The Cancer Foundation of Western Australia and the National Health and Medical Research Council of Australia. This research was supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government, and also by grants from the Radium Hospital Foundation and the Fredriksen Foundation for Ovarian Cancer Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.